Table 1.
Pairwise correlation between ratios estimates for the loop design
Method 1 | Method 2 | T3/T1 | T4/T1 | T5/T1 | T6/T1 | Mean |
lmbr/lmbr_dye | limmaQual | 0.9966 | 0.9998 | 0.9648 | 0.9909 | 0.9880 |
lmbr/lmbr_dye | anovaFix | 0.9899 | 0.9913 | 0.9721 | 0.9856 | 0.9848 |
lmbr/lmbr_dye | anovaMix | 0.9829 | 0.9751 | 0.9420 | 0.9549 | 0.9637 |
limmaQual | anovaFix | 0.9889 | 0.9918 | 0.9298 | 0.9775 | 0.9720 |
limmaQual | anovaMix | 0.9810 | 0.9758 | 0.8950 | 0.9467 | 0.9496 |
anovaFix | anovaMix | 0.9936 | 0.9847 | 0.9726 | 0.9694 | 0.9801 |
Correlation between ratios estimated by each pair of applied methods (column 1 and column 2) for a loop design. Each ratio corresponds to a time dependent change in expression as compared to the first time point (T1). The last column corresponds to the mean correlation of the 4 estimates. Since the loop design is balanced with respect to the dyes, the results for lmbr and lmbr_dye were the same (see 'Methods' section), which is why they are not treated differently.