Table 7.

Concentration (copies per cell) of the control clones spiked

Spike No.	Spike Mix 1	Spike Mix 2	Spike Mix 3	Spike Mix 4	Spike Mix 5	Spike Mix 6	Spike Mix 7	Reference Mix
1, 2	10,000	0	0.1	1	10	100	1,000	100
3, 4	1,000	10,000	0	0.1	1	10	100	100
5, 6	100	1,000	10,000	0	0.1	1	10	100
7, 8	10	100	1,000	10,000	0	0.1	1	100
9, 10	1	10	100	1,000	10,000	0	0.1	100
11, 11a	0.1	1	10	100	1,000	10,000	0	100
12, 13	0	0.1	1	10	100	1,000	10,000	100

From the total of 14 arrays, 7 were hybridized with the respective spike mixes labeled in Cy5 against the reference mix labeled in Cy3. The remaining 7 arrays were hybridized with the spike mixes labeled in Cy3 against the reference mix labeled in Cy5. Spike 11a was removed from analysis due to quality issues (Allemeersch et al., 2005 [17]).