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. 2008 Jan 28;6:5. doi: 10.1186/1477-5956-6-5

Table 1.

Mycobacterium avium subsp paratuberculosis proteins used in this study

Protein namea Concentration (mg/ml)b Gene sizec Amino acidsd Predicted function
MAP sonicate 1.0 NAe NA NA
MAP0075 0.18 423 140 conserved small membrane protein
MAP0087-his 1.0 717 238 probable secreted protein
MAP0105c 1.24 1058 352/889 hypothetical protein
MAP0182c 0.67 411 136 conserved hypothetical protein
MAP0216 1.66 1028 342/347 antigen 85A, mycolyltransferase
MAP0261c 0.49 465 154/161 19 kDa protein
MAP0389 2.04 947 315/337 diarylpropane peroxidase (EC 1.11.1.14) [Nostoc]
MAP0736 5.73 744 247 hypothetical protein
MAP0852 10.4 546 181 no BLAST hits
MAP0853 4.04 660 219 no BLAST hits
MAP0855 0.34 926 308/314 no BLAST hits
MAP0857c 0.70 318 105 hypothetical protein
MAP0858 0.44 537 178/182 no BLAST hits
MAP0859c 0.66 609 202 Mycobacterium phage L5
MAP0860c 0.04 885 294/296 no BLAST hits
MAP0861 0.43 342 113 no BLAST hits
MAP0862 0.48 989 329/360 no BLAST hits
MAP0863 0.66 675 224 no BLAST hits
MAP0864 7.42 426 141 no BLAST hits
MAP0865 0.08 1272 423 Hypothetical 30.9 kDa protein
MAP0866 3.07 804 267 integrase
MAP0904 0.81 371 124/246 conserved hypothetical protein
MAP0961c 0.18 719 239/352 hypothetical protein
MAP1087 0.43 441 146 prob. peptide transp. system permease, N-terminal
MAP1121c 0.32 498 165 putative substrate binding protein
MAP1174c 1.64 756 251 glucose-6-phosphate 1-dehydrogenase
MAP1204 2.61 735 244 putative exported p60 protein homologue
MAP1233 0.04 723 240 conserved hypothetical protein
MAP1345 0.69 600 199 no BLAST hits
MAP1388 0.08 396 131 no BLAST hits
MAP1416c 0.45 666 221 no BLAST hits
MAP1417c 0.54 435 144 no BLAST hits
MAP1609c 0.05 987 328/330 antigen 85B, mycolyltransferase
MAP1636c 1.12 425 141/157 no BLAST hits
MAP1643 0.45 2277 759/762 isocitrate lyase, [beta] module
MAP1655c 0.93 381 126 hypothetical protein
MAP1730c 0.26 1023 340 putative ATP/GTP-binding protein
MAP1931c 0.32 1104 367 anthranilate phosphoribosyltransferase
MAP2077c 1.66 330 109 hypothetical protein
MAP2116c 0.27 1269 422 cell invasion protein
MAP2121c 7.14 887 295/307 major membrane protein I
MAP2121c-his 0.99 924 307 major membrane protein I
MAP2151 2.79 438 145 no BLAST hits
MAP2155 9.57 312 103 similar to IS6110
MAP2156 0.59 963 320 putative transposase GEN: X52471)
MAP2157 0.38 1221 406 IS900
MAP2158 2.40 582 193 no BLAST hits
MAP2182c 0.26 435 144 conserved hypothetical protein
MAP2231 0.31 1422 473 PKS-associated protein, unknown function
MAP2360c 0.13 534 177 hypothetical protein
MAP2380 2.00 600 200/550 acyl-CoA synthase
MAP2657 0.26 651 216 putative oxidoreductase
MAP2663c 0.03 510 169 putative integral membrane protein
MAP2676c 1.30 354 117 hypothetical protein
MAP2734 0.37 1341 446 putative dioxygenasesdiooxygenases
MAP2740 0.24 666 221 putative membrane protein
MAP2751 0.70 582 193 no BLAST hits
MAP2753 0.17 309 103/252 hypothetical protein
MAP2761c 0.03 692 230/238 no BLAST hits
MAP2762c 1.45 425 141/146 no BLAST hits
MAP2764c 0.10 290 96/149 no BLAST hits
MAP2765c 0.06 539 179/396 no BLAST hits
MAP2767c 0.42 473 157/183 no BLAST hits
MAP2963c 0.88 1499 499/874 no BLAST hits
MAP3084c-his 1.0 684 227 probable secreted protein
MAP3121 0.38 855 284 enoyl-CoA hydratase/isomerase superfamily
MAP3129 0.09 404 134/141 hypothetical protein
MAP3131 0.30 1096 365/942 conserved large membrane protein
MAP3155c 1.22 273 90 hypothetical protein
MAP3434 0.18 690 230/330 possible membrane protein
MAP3437c 0.29 843 279/280 no BLAST hits
MAP3531c 0.28 1034 344/352 antigen 85C, mycolytransferase
MAP3734c 0.19 1559 519/593 putative ABC transporter ATP-binding protein
MAP3735c 0.42 1181 393/429 part of heavy metal tolerance protein
MAP3743 0.85 998 332/348 hypothetical protein
MAP3751 0.11 879 293/979 conserved large membrane protein
MAP3753 0.64 1353 450 hypothetical protein
MAP3761c 0.07 729 242 conserved membrane protein
MAP3771 0.02 294 97 50S ribosomal protein L31
MAP3817c 0.65 939 312 no BLAST hits
MAP3833c 0.13 626 208/260 hypothetical protein
MAP3840 0.10 1872 623 70 kD heat shock protein, chromosome replication
MAP3902c 0.15 522 173 Serine/threonine kinase (EC 2.7.1.37) [Myc...
MAP3903c 0.42 531 176 Serine/threonine kinase (EC 2.7.1.37) [Myc...
MAP3954 0.05 279 92 conserved hypothetical protein
MAP4014 0.21 951 316 probable 4-amino butyrate transporter, N-terminal
MAP4025 0.32 854 284/325 cytochrome c-type biogenesis protein
MAP4129 0.26 965 321/336 ABC transporter
MAP4198 0.13 1283 427/441 SecY subunit of preprotein translocase
MAP4207c 5.28 686 228/231 probable ATP-binding transport protein
MAP4199 0.03 540/546 179/181 probable adenylate kinase
MAP4228 0.05 222 73 initiation factor IF-1

aProtein name corresponds to the genome project designation (Li et al., 2005). A second designation is included if known.

bProtein concentration is reported in as measured by Nanodrop spectrometry at 280nm.

cGene size is reported in base pairs (bp). In cases where only a portion of the M. avium subsp paratuberculosis gene was cloned, the number of bp present in the clone is reported first followed by the number of bp in the full-length M. avium subsp paratuberculosis gene.

dNumber of M. avium subsp paratuberculosis amino acids present in the fusion protein. In cases where only a portion of the M. avium subsp paratuberculosis protein is represented, the number of amino acids present is listed first followed by the total number of M. avium subsp paratuberculosis amino acids in the full-length protein.

eNA is not applicable.