Table 2.
Spot intensities of proteins present on the array.a
| Cow 5902 | Cow 5904 | ||||||||
| Spot Locb | Protein | Day 0 | Day 70 | Day 194 | Day 321 | Day 0 | Day 70 | Day 194 | Day 321 |
| A7 | MAP0075 | 0 | 4.472 | 21.431 | 21.122 | 0 | 0 | 27.612 | 21.36 |
| A12 | MAP0087-his | 0.978 | 0 | 0 | 0 | 1.112 | 1.882 | 0 | 1.228 |
| A5 | MAP0105c | 0 | 6.929 | 3.867 | 5.096 | 1.186 | 2.321 | 10.106 | 1.813 |
| B5 | MAP0182c | 0 | 5.407 | 0 | 1.924 | 1.177 | 2.409 | 1.617 | 22.366 |
| C4 | MAP0216 | 0.652 | 3.779 | 1.296 | 1.395 | 2.175 | 3.444 | 0 | 0 |
| G4 | MAP0261c | 2.395 | 2.287 | 3.487 | 5.668 | 1.097 | 3.146 | 2.397 | 2.496 |
| E8 | MAP0389 | 0 | 7.809 | 3.163 | 1.817 | 0 | 1.533 | 16.751 | 5.529 |
| F8 | MAP0736 | 0.734 | 0 | 0 | 0 | 0 | 0 | 1.446 | 0 |
| A1 | MAP0852 | 2.257 | 3.747 | 2.908 | 1.147 | 2.564 | 4.661 | 2.709 | 1.537 |
| B1 | MAP0853 | 2.144 | 3.734 | 5.095 | 8.497 | 1.961 | 4.158 | 3.239 | 2.649 |
| C1 | MAP0855 | 2.538 | 3.387 | 4.552 | 7.898 | 1.716 | 2.248 | 2.132 | 0 |
| D1 | MAP0857c | 2.709 | 6.445 | 21.867 | 37.718 | 1.045 | 1.602 | 23.425 | 15.825 |
| E1 | MAP0858 | 3.005 | 3.882 | 1.775 | 3.999 | 1.978 | 0 | 0 | 0 |
| F1 | MAP0859c | 2.821 | 4.137 | 2.87 | 3.745 | 1.909 | 0 | 2.803 | 0 |
| G1 | MAP0860c | 2.996 | 0 | 5.531 | 12.743 | 1.592 | 0 | 7.669 | 6.676 |
| H1 | MAP0861 | 5.074c | 0 | 3.407 | 3.053 | 1.546 | 4.05 | 3.111 | 3.52 |
| A2 | MAP0862 | 1.795 | 40.036 | 16.196 | 7.997 | 2.796 | 11.451 | 30.918 | 12.422 |
| B2 | MAP0863 | 1.518 | 5.524 | 3.174 | 4.592 | 2.057 | 4.891 | 3.274 | 1.473 |
| C2 | MAP0864 | 1.255 | 3.44 | 1.781 | 1.726 | 2.016 | 3.56 | 0 | 0 |
| D2 | MAP0865 | 1.909 | 18.708 | 32.138 | 44.846 | 1.317 | 4.386 | 31.361 | 35.251 |
| E2 | MAP0866 | 2.346 | 7.051 | 2.67 | 7.116 | 1.315 | 3.561 | 4.052 | 6.009 |
| G8 | MAP0904 | 1.678 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| G5 | MAP0961c | 1.797 | 14.366 | 26.5 | 37.546 | 0.78 | 2.103 | 33.046 | 42.877 |
| A11 | MAP1087 | 0.753 | 53.179 | 51.634 | 70.968 | 0.874 | 33.469 | 75.392 | 68.964 |
| B11 | MAP1121c | 0.758 | 2.438 | 0 | 2.094 | 0.699 | 3.364 | 0 | 1.848 |
| A10 | MAP1174c | 0 | 2.301 | 3.155 | 4.411 | 0.623 | 1.921 | 5.81 | 4.955 |
| C11 | MAP1204 | 0.78 | 40.8 | 43.856 | 55.046 | 0 | 28.367 | 61.122 | 58.985 |
| F2 | MAP1233 | 2.568 | 12.375 | 22.507 | 32.493 | 1.932 | 3.212 | 24.157 | 30.447 |
| C5 | MAP1345 | 0 | 3.339 | 1.861 | 1.018 | 1.147 | 0 | 3.413 | 2.389 |
| D5 | MAP1388 | 0 | 2.281 | 0 | 0 | 0.429 | 2.835 | 0 | 0 |
| E5 | MAP1416c | 0 | 1.647 | 0 | 0 | 0.791 | 3.791 | 0 | 0 |
| F5 | MAP1417c | 1.052 | 14.882 | 4.095 | 6.212 | 0.955 | 3.979 | 7.614 | 3.521 |
| D4 | MAP1609c | 0.733 | 3.916 | 0 | 0 | 1.003 | 5.292 | 0 | 0 |
| G2 | MAP1636c | 3.286 | 6.944 | 13.249 | 17.272 | 1.701 | 0 | 17.226 | 12.06 |
| B4 | MAP1643 | 0 | 4.155 | 0 | 1.534 | 1.827 | 0 | 0 | 0 |
| H8 | MAP1655c | 2.788 | 0 | 0 | 0 | 0 | 2.049 | 0 | 0 |
| G6 | MAP1730c | 1.147 | 29.831 | 43.872 | 52.35 | 0 | 12.272 | 57.799 | 57.47 |
| B10 | MAP1931c | 0 | 2.917 | 0 | 2.217 | 0.511 | 2.886 | 2.077 | 3.339 |
| H5 | MAP2077c | 3.833 | 0 | 2.784 | 9.443 | 1.385 | 0 | 3.63 | 9.221 |
| D11 | MAP2116c | 1.418 | 29.88 | 33.215 | 42.633 | 0.568 | 11.24 | 51.336 | 47.918 |
| F4 | MAP2121c | 1.76 | 4.158 | 1.373 | 1.513 | 0.969 | 3.005 | 0 | 0 |
| B12 | MAP2121c-his | 1.247 | 0 | 0 | 0 | 0.889 | 2.172 | 21.226 | 2.155 |
| H2 | MAP2151 | 4.136 | 1.919 | 12.688 | 21.897 | 1.571 | 3.402 | 24.619 | 20.889 |
| F12 | MAP2155 | 2.434 | 0 | 1.385 | 0 | 1.251 | 2.039 | 0 | 2.105 |
| G12 | MAP2156 | 3.731 | 2.327 | 0 | 2.12 | 1.357 | 2.647 | 5.42 | 5.113 |
| H6 | MAP2157 | 3.504 | 0 | 0 | 0 | 0.751 | 1.532 | 1.51 | 2.683 |
| A3 | MAP2158 | 1.403 | 3.487 | 4.2 | 3.396 | 3.187 | 1.704 | 6.682 | 2.204 |
| A9 | MAP2182c | 0 | 19.101 | 7.349 | 8.933 | 0 | 0 | 27.935 | 12.096 |
| E11 | MAP2231 | 1.846 | 3.732 | 11.54 | 15.671 | 1.166 | 0 | 17.203 | 18.933 |
| B9 | MAP2360c | 0 | 3.168 | 0 | 0 | 0 | 2.766 | 0 | 0 |
| C10 | MAP2380 | 0.772 | 2.139 | 1.198 | 0 | 0 | 0 | 1.968 | 3.225 |
| D10 | MAP2657 | 0 | 22.372 | 8.29 | 3.68 | 0 | 15.937 | 39.178 | 25.032 |
| B7 | MAP2663c | 0 | 9.594 | 3.661 | 2.248 | 0.868 | 0 | 4.9 | 2.717 |
| A6 | MAP2676c | 0 | 3.291 | 1.406 | 0 | 0 | 0 | 2.036 | 0 |
| D7 | MAP2734 | 2.726 | 9.109 | 11.031 | 9.269 | 0 | 0 | 27.935 | 18.79 |
| C7 | MAP2740 | 0 | 1.736 | 6.447 | 18.061 | 0.507 | 0 | 6.295 | 10.804 |
| B6 | MAP2751 | 0 | 3.157 | 1.101 | 2.373 | 0.8 | 0 | 1.729 | 2.206 |
| C6 | MAP2753 | 0 | 1.909 | 0 | 2.176 | 0.624 | 0 | 1.701 | 2.585 |
| D6 | MAP2761c | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E6 | MAP2762c | 0 | 0 | 0 | 0 | 0 | 2.397 | 0 | 0 |
| B3 | MAP2764c | 1.472 | 2.739 | 1.969 | 5.874 | 2.13 | 5.442 | 2.039 | 1.955 |
| H11 | MAP2765c | 4.816 | 0 | 0 | 0 | 0 | 4.799 | 0 | 0 |
| C3 | MAP2767c | 1.257 | 3.507 | 2.303 | 7.019 | 1.982 | 4.347 | 1.92 | 2.722 |
| F6 | MAP2963c | 0 | 0 | 0 | 0 | 0 | 2.204 | 0 | 0 |
| C12 | MAP3084c-his | 1.313 | 0 | 0 | 0 | 0.999 | 2.026 | 0 | 1.982 |
| E10 | MAP3121 | 1.079 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| C9 | MAP3129 | 1.406 | 2.366 | 0 | 1.206 | 0 | 0 | 1.731 | 2.865 |
| E7 | MAP3131 | 0 | 0 | 0 | 0 | 0 | 0 | 1.404 | 1.713 |
| G11 | MAP3155c | 2.783 | 2.553 | 2.725 | 3.841 | 1.435 | 2.237 | 2.226 | 4.865 |
| F7 | MAP3434 | 0.832 | 1.401 | 8.312 | 19.522 | 0.508 | 0 | 33.289 | 14.418 |
| D3 | MAP3437c | 1.674 | 3.321 | 1.29 | 1.587 | 1.359 | 1.965 | 0 | 0 |
| E4 | MAP3531c | 0.926 | 7.373 | 2.472 | 4.313 | 0.558 | 2.834 | 1.374 | 0 |
| G7 | MAP3734c | 1.085 | 2.238 | 2.719 | 17.809 | 0 | 0 | 10.914 | 20.81 |
| E3 | MAP3735c | 1.944 | 4.897 | 5.078 | 18.618 | 1.326 | 3.368 | 3.887 | 4.502 |
| F3 | MAP3743 | 2.113 | 23.874 | 16.282 | 16.773 | 1.28 | 6.953 | 21.309 | 22.067 |
| H7 | MAP3751 | 2.991 | 0 | 0 | 0 | 0 | 0 | 2.499 | 3.033 |
| D9 | MAP3753 | 0 | 2.912 | 7.043 | 3.212 | 0 | 0 | 18.95 | 15.247 |
| A8 | MAP3761c | 0 | 2.473 | 14.931 | 23.178 | 0 | 0 | 18.02 | 21.264 |
| G3 | MAP3771 | 2.81 | 2.229 | 1.344 | 2.528 | 0.953 | 1.997 | 0 | 0 |
| H3 | MAP3817c | 3.836 | 2.526 | 10.298 | 22.826 | 1.101 | 1.552 | 15.736 | 19.901 |
| E9 | MAP3833c | 1.078 | 0 | 2.07 | 2.755 | 0 | 0 | 3.676 | 4.636 |
| A4 | MAP3840 | 0.823 | 4.968 | 3.842 | 4.521 | 1.403 | 0 | 10.083 | 0 |
| F9 | MAP3902c | 1.095 | 0 | 0 | 0 | 0 | 2.376 | 0 | 0 |
| G9 | MAP3903c | 1.755 | 1.355 | 0 | 1.027 | 0 | 1.772 | 0 | 0 |
| H9 | MAP3954 | 3.426 | 0 | 0 | 1.969 | 0 | 7.584 | 1.612 | 3.629 |
| F11 | MAP4014 | 2.266 | 2.035 | 5.415 | 8.98 | 0.545 | 0 | 5.293 | 8.043 |
| F10 | MAP4025 | 1.474 | 25.139 | 34.876 | 39.022 | 0 | 13.413 | 49.655 | 47.012 |
| B8 | MAP4129 | 0 | 1.489 | 0 | 1.363 | 0 | 0 | 1.435 | 2.585 |
| C8 | MAP4198 | 0 | 1.536 | 1.517 | 6.817 | 0 | 0 | 5.431 | 6.175 |
| G10 | MAP4199 | 1.919 | 1.688 | 0 | 0 | 0.821 | 2.339 | 0 | 0 |
| D8 | MAP4207c | 1.981 | 1.691 | 1.872 | 1.364 | 0 | 0 | 3.699 | 5.632 |
| H10 | MAP4228 | 4.373 | 0 | 0 | 0 | 0 | 3.122 | 0 | 0 |
| H4 | MBP-LacZ | 4.128 | 0 | 0 | 1.722 | 1.303 | 0 | 0 | 0 |
| D12 | PBS bufferd | 1.676 | 0 | 1.236 | 0 | 0.857 | 2.322 | 0 | 2.003 |
| E12 | Sonicatee | 2.673 | 0 | 0 | 0 | 1.287 | 1.936 | 2.963 | 3.523 |
| H12 | Sonicate | 5.01 | 1.359 | 0 | 0 | 1.366 | 4.337 | 3.002 | 4.472 |
aListed are the intensity values for each spot arranged by ascending MAP number. Measurements were made using a consistent 1804 pixel area circle to record spot density using the Adobe Photoshop CS3 Extended application.
bLoc is location on the dot blot array.
cThe strongest intensity for each time point is shown in bold.
dPBS buffer in spot D12 represents the spotting buffer only control.
eSonicate is a sonicated whole cell lysate of M. avium subsp paratuberculosis.