Figure 2.

The phosphorylated H3 antibody is specific for phosphorylated micronuclear H3^F. (A) Total proteins from either macro- (lane 1) or micronuclei (lane 2) of logarithmically growing Tetrahymena cells were resolved on a 12% SDS/PAGE gel, transferred to a nitrocellulose membrane, and probed by either phosphorylated H3 antibody (Left) or general H3 antibody (Right). (B) HPLC-purified H3 from micronuclei of logarithmically growing Tetrahymena cells was incubated in the presence (lane 2) or absence (lane 1) of alkaline phosphatase (AP) and subjected to immunoblotting by either phosphorylated H3 antibody (Upper) or general H3 antibody (Lower).