Figure 3.

H3 phosphorylation is temporally correlated with mitosis in Tetrahymena. Logarithmically growing Tetrahymena cells were stained with the phosphorylated H3 antibody (B, D, F, H, and J) and the DNA-specific dye DAPI (A, C, E, G, and I). In A and B, a random population of cells is shown in low magnification. The closed arrows denote mitotic micronuclei; the open arrows denote interphase micronuclei, and the arrowheads denote macronuclei. The bar represents 10 μm. In C, D, E, F, G, H, I, and J, cells with micronuclei in interphase, mitosis, and S phase are shown in high magnification. The arrows denote micronuclei. The bar represents 10 μm.