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. 2007 Dec 21;8:119. doi: 10.1186/1471-2199-8-119

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Copyright © 2007 Mickle et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of inducing cdc18* on DNA content and cell cycle division arrest. cdc18* was induced in the wild-type (green), cds1Δ (blue), and rad3Δ (red) strains by removal of thiamine at 0 hours. (A) Fluorescence microscopy of cells 25 hours after cdc18* induction. Cells are stained with DAPI to show nuclei. Arrows show binucleate rad3Δ cells, which are evidence for active cell division. (B) Histogram plots of DNA content determined by flow cytometry for 0, 17, 21, and 25 hours after removal of thiamine. (C) Density plots of DNA content (fluorescence intensity, x-axis) versus cell size (forward scatter, y-axis) at 25 hours. Wild-type and cds1Δ cells are greatly elongated with high DNA content whereas rad3Δ cells are shorter and have lower DNA content due to cell division during re-replication. The insets show the number of cells out of 10,000 represented by pixels of the indicated color.