Figure 7.
Stimulation of O2 evolution of PSII membrane fragments caused by binding of Cah3. (A) Immunoblot analysis of PSII membrane fragments from the wt and the cia3 mutant (cia3). The recombinant Cah3 protein was loaded as a positive control (Cah3). (B) Immunoblot analysis of PSII(Cah3−) after reconstitution with recombinant Cah3 protein, followed by centrifugation to obtain pellet (P) and soluble (S) fractions. The antibodies used were against Cah3, PsbO and CAII. Lanes cia3, wt and P were loaded with 7 μg of Chl and Cah3 along with 1.2 μg Cah3 protein.