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. 2008 Jan 31;27(5):770–781. doi: 10.1038/emboj.2008.14

Figure 2.

Figure 2

Apoptosis is increased in FA VZ. (A) DAPI and cleaved-caspase-3 immunostaining are shown in a fanca−/− E14.5 neocortex section. Arrows pointed out the apoptotic cells in the VZ. Nonspecific staining was observed on blood vessels (stars) and on meninges (dotted lines). Colocalization of pycnotic nuclei and cleaved-caspase-3 staining is shown in the enlargement of the white box (A′). White arrows pointed out the isolated apoptotic cells and red arrows pointed out a doublet of apoptotic cells. (B) Quantifications of pycnotic nuclei and (C) cleaved-caspase-3-positive cells in the VZ of E13.5 and E14.5 embryonic brains show a significant increase of apoptosis in fanca−/− and fancg−/− compared with fanca+/+ and fancg+/+ controls. (D) Apoptotic cells in the fancg−/− neocortex express nestin, an NSPC marker. The number of mice is indicated within the bars. V, ventricle. Scale bars, 10 μm. *P<0.05; ***P<0.005.