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. 2008 Jan 31;27(5):770–781. doi: 10.1038/emboj.2008.14

Figure 7.

Figure 7

Exhaustion of the NSPC pool in the SVZ of FA mice is accentuated with ageing. (A) Quantification of NG2/Ki67-double-positive cells in the adult SVZ shows decreased level of proliferating progenitors in the SVZ of young (2–3 months) and old (10–12 months) fanca−/− adults compared with fanca+/+ age-matched controls. (B) BrdU-label-retaining cells in the adult SVZ, recognized as slowly cycling neural stem cells, have a reduced level in the SVZ of young (2–3 months) and old (10–12 months) fanca−/− adults compared with fanca+/+ age-matched controls. (C) The NSPC pool was determined by culturing freshly harvested SVZ in a semi-solid medium that allows discrimination between neural stem cell-derived colonies and neural progenitor-derived colonies. The number of neural progenitor-derived colonies was decreased in the SVZ of young (2–3 months) fanca−/− and fancg−/− adults compared with fanca+/+ and fancg+/+ age-matched controls. Both neural stem cell- and progenitor-derived colonies were profoundly reduced in old fancg−/− mice. Data were obtained with 2–6 mice, as indicated within or below (n=) the bars, representing in panels A and B from 1908 to 2894 nuclei (except the number of nuclei for NG2/Ki67 in old fanca−/− mice, which was 798). *P<0.05; **P<0.01; ***P<0.005.