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. 2008 Feb 7;27(5):736–747. doi: 10.1038/emboj.2008.17

Figure 1.

Figure 1

Antagonistic relationship of PABPC1 and the EJC in stop codon definition. (A) Schematic representation of the 4boxB/4MS2 and 4MS2/4boxB reporters. White and black boxes represent exons and introns of the β-globin gene, respectively. BoxB and MS2 sites are shown as white boxes or black loops, respectively. (B) Tethering of PABPC1 and the EJC protein Y14. HeLa cells were transfected with the indicated 4boxB/4MS2 or 4MS2/4boxB constructs and the plasmids expressing the MS2–PABPC1 and λNV5–Y14 fusion proteins as indicated. Total RNA samples were analysed by northern blotting. The levels of the 4boxB/4MS2 and the 4MS2/4boxB reporter mRNAs were normalized to the control β-globin reporter (ctrl). For each reporter, mRNA levels of the reporter without any tethered factor (MS2 protein and λN peptide alone) were set as 1.0 (lanes 1 and 2). Values were calculated from three independent experiments, error bars represent standard deviations.