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. 2008 Feb 14;27(5):748–757. doi: 10.1038/emboj.2008.21

Figure 2.

Figure 2

Deletion of ESTs or YKU70 resulted in the cytoplasmic accumulation of the TLC1 RNA. (A) FISH against TLC1 RNA in WT (BY4742), est1, est2 or est3 strain. Scale bar=1 μm. (B) FISH against TLC1 RNA in yku70 and a tlc1-Δstemyku strains. Scale bar=1 μm. (C) TLC1 RNA expression level measured by qPCR in the various strains. TLC1 RNA level was normalized against that of U1 snRNA and compared with the RNA levels in the WT strain (BY4742). (D) Level of TMG-capped TLC1 RNA in the various strains measured by immunoprecipitation with an anti-TMG antibody followed by semiquantitative RT–PCR amplification of the TLC1 RNA. Numbers indicate PCR cycles. T (total): RNA from total extract; B (bound): RNA immunoprecipitated with anti-TMG antibody. Anti-TMG immunoprecipitation and RT–PCR amplification (30 cycles) of ACT1 mRNA served as a negative control.