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. 2008 Mar 11;6(3):e58. doi: 10.1371/journal.pbio.0060058

Figure 2. Pioneer Neurons Are Not Required to Maintain the Larval Axonal Tracts.

Figure 2

(A) Wild-type expression of dMP2-GAL4 in dMP2 neurons. Expression in A8/A9 is weak or absent.

(B) No dMP2 neurons remain at the end of embryogenesis following expression of the cell death activators rpr and hid from dMP2-GAL4.

(C) Wild-type expression of oddGAL4 in dMP2 and MP1 pioneer neurons in air-filled tracheae embryos at the end of embryogenesis.

(D) Complete absence of dMP2 and MP1 pioneer neurons at the end of embryogenesis following expression of rpr and hid from oddGAL4.

(E–G) Fasciclin II staining of larval longitudinal connectives is unaffected by the ablation of dMP2 neurons alone (F) or dMP2 and MP1 neurons (G) in late embryos.

Genotypes: (A) dMP2-GAL4/UAS-nmEGFP; (B and F) UAS-hid,UAS-rpr/Y; dMP2-GAL4/UAS-nmEGFP; (C) oddGAL4,UAS-GFP/+; (D and G) UAS-hid,UAS-rpr/Y; oddGAL4,UAS-GFP/+; and (E) w1118.