(A) Alignment of 5′ regions of Amyrel in D. melanogaster (mel), D. ananassae (ana), D. pseudoobscura (pse), and D. subobscura (sub). Shading indicates invariant nucleotides. Long conserved sequences are numbered i, ii, and iii (see text); iv is the putative TATA box. clustalw alignment was corrected manually. (B) Alignment of the 3′ regions (except D. pseudoobscura, which was not available). The putative polyadenylation site is boxed.