Figure 6.

RT-PCR analysis of Amyrel and Amy in D. melanogaster. The primers used are indicated in Table 1. Each time point was repeated on several individuals, one of which was representative and was selected for both genes. The sizes of amplified cDNAs are 344 bp for Amyrel and 352 bp for Amy. (E, embryo; L1, larva, first instar; L2, larva, second instar; 72h, L2/L3 molt time at 25°C; wL, wandering larva; wp, white pupa; bp, black pupa; em, emerging adult; ++, control (96-h larva) treated with DNase and RTase; +−, control treated with DNase and without RTase; − −, control with no DNase and no RTase; −, negative control.)