Table 1.
List of the primers used in this study
| Primer name | Sequence (strand) | Position | Use |
|---|---|---|---|
| Amyrel1 | GAGAACATCATTTCGGCGG (+) | 196/214 | (RT)PCR Amyrel |
| Amyrel2 | TGGTCGAGACCCTTGAGGC (−) | 521/539 | (RT)PCR Amyrel |
| Rev1230 | TTGCTGCCRTTRTCCCACC (−) | 1273/1291 | PCR Amy/Amyrel |
| Haerel279 | TTCCAGGTGCAACAGTGCG (+) | 493/511 | inverse PCR Amyrel |
| Haerel855 | CTCATCACCCGACTGGTGC (−) | 1072/1090 | inverse PCR Amyrel |
| Haerel1024 | GAACGCCGTTCGGGATACG (+) | 1241/1259 | inverse PCR Amyrel |
| Haerel58 | TCGTCCACTTGTTCGAGTG (+) | 95/113 | inverse PCR Amyrel |
| Haerel5 | CCACCAATGGGGATTGTGC (−) | 60/78 | inverse PCR Amyrel |
| Intr1U | GTTCACCTCTTCGAGTGG (+) | 94/111 | RT-PCR Amy |
| Mel2 | AGTCCAGCGAGGAGTAGGG (−) | 427/445 | RT-PCR Amy |
Positions are numbered from the translation start. They were chosen with help of the program amplify 1.2 by Bill Engels. Some of them were designed to amplify Amyrel or Amy specifically; other primers were compatible with both classical and Amyrel genes. Primer Intr1U also matches to Amyrel.