Figure 1.

In vitro proliferation of T_reg and T_conv cells after stimulation with the CD28-SA mAb JJ316 in the presence of different amounts of rhIL-2. (a)[³H]thymidine incorporation by 10⁴ freshly purified T_reg cells (black bars) or T_conv cells (white bars) stimulated for 3 days with the indicated amounts of CD28-SA JJ316 and rhIL-2. The bars indicate means of triplicate cultures ± SD. (b) CFSE-labelled T_reg cells (left) and CFSE-labelled T_conv cells (right) were cultured for 3 days together with irradiated splenic APCs in the presence (black line) or absence (grey shadow) of the CD28-SA added in solution.