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. 2006 Dec 1;119(4):488–498. doi: 10.1111/j.1365-2567.2006.02460.x

Figure 3.

Figure 3

Tumour necrosis factor (TNF)-α secretion by activated plasmacytoid dendritic cells (PDCs) is impaired by tacrolimus pretreatment. (a) PDCs were incubated during 48 hr in the presence of interleukin (IL)-3 and tacrolimus at the dose indicated. After this pretreatment, dinucleotide–oligodeoxynucleotide (CpG–ODNs) were added. After the additional 48 hr of incubation, supernatants were recovered. Indicated cytokines were determined either by enzyme-linked immunosorbent assay or cytometric bead array (CBA). Each symbol represents an individual sample. Significant differences are indicated (*P < 0·05, paired t-test). (b) Cells were pretreated with the indicated dose of tacrolimus for 12 hr. Then CpG–ODN were added and cells incubated further during 6–8 hr before mRNA was extracted. The expression of TNF-α mRNA is shown as the relative proportion (TNF-α/β2 m) normalized to the maximum level of stimulation (CpG-activated non-pretreated PDCs). The mean ± SD of two independent experiments is shown.