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. 2006 Dec 1;119(4):529–540. doi: 10.1111/j.1365-2567.2006.02468.x

Figure 3.

Figure 3

Humoral immune response of BALB/c mice 15 days after the last LmSIR2 injection. Groups of three mice were immunized i.p. with LmSIR2 as indicated in Materials and methods. (a) Levels of total IgG1, IgG2a, IgG2b and IgG3 in the sera of LmSIR2-treated and untreated BALB/c mice were quantified by ELISA in comparison to standard curves using purified mouse IgG1, IgG2a, IgG2b and IgG3, respectively. The asterisk indicates a statistically significant difference (P < 0·05) between untreated and LmSIR2-treated mice. (b) IgG1 and IgG2a antibodies levels in the sera of LmSIR2-treated BALB/c mice reacting against LmSIR2, L. infantum total extract, keyhole limpet hemocyanin (KLH) and myoglobulin (MYO) were determined by ELISA. Sera were diluted 1/100 in PBS−1% gelatin. The data represent the mean and the standard deviations of three animals analysed individually and are representative of two independent experiments.