Figure 5.

Lung histopathology from the naive, vaccinated, and antibody-treated CD4^–/– mice showed substantially worsening pathology only in vaccinated mice that were depleted of Thy-1.2⁺ cells and not in immunized CD4^–/– mice that had been depleted of CD8⁺, TCR-γδ⁺ and NK1.1⁺ cells. Lung tissue was obtained from each group at the time of death, fixed in formalin, and embedded in paraffin. The lung sections were stained with haematoxylin & eosin (a–d) or with Ziehl–Neelsen acid-fast stain (e–h) and evaluated by light microscopy. Stained lung sections from naive (a, e), mc²6030-vaccinated (b, f), mc²6030-vaccinated and anti-CD8, NK1.1, and TCR-γδ mAb-treated (c, g), and mc²6030-vaccinated and anti-Thy-1.2 mAb-treated (d, h) mice are shown above.