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. 2007 Feb;120(2):242–250. doi: 10.1111/j.1365-2567.2006.02495.x

Figure 2.

Figure 2

Knocked down MEKK3 expression in the macrophage line Raw264.7. (a–d) Raw264.7 cells (a) and lentivirus-infected Raw264.7 cells that express Luci-siRNA (b), Mekk3-siRNA15 (c), or Mekk3-siRNA35 (d) after sorting for GFP-positive cells followed by FACS analysis. (e) Equal amounts of cell lysates prepared from the cells described above in (a–d) were analysed by immunoblotting using antibodies against MEKK3, ERK2, p38 and β-actin, as indicated. One representative experiment of three is shown.