Figure 2.

Proliferative responses of the peptide-specific T cell lines of Can f 1 skinprick test-positive allergic (a) and non-allergic control (b) subjects upon stimulation with p33–48 and p107–122. Peripheral blood mononuclear cells were isolated from heparinized blood samples and stimulated with p33–48 or with p107–122. After the first stimulation cycle, specificity of the cultures was determined using the thymidine incorporation test. Lines with stimulation indices ≥ 2 were re-stimulated for further study. The reactivity of these T cell lines to the peptide used in the induction was examined at 0–50 µm. Results are presented as mean counts per minute ± SEM.