Figure 2.

Cytokine production of bone marrow dendritic cells (BM-DCs) during maturation and phenotype change.(a) Immature BM-DCs of B6 mice were stimulated by lipopolysaccharide (LPS) (1 µg/ml) or LPS (1 µg/ml) with αCD40 (5 µg/ml) to induce exhausted or stable mature phenotypes. During the culture, the culture media were collected and exchanged with fresh medium at 6 hr, 24 hr and 30 hr, and the amount of IL-12p40 secreted by the DCs in the cultures was measured by enzyme-linked immunosorbent assay (ELISA) for the periods 0–6 hr and 24–30 hr. (b) BM-DCs stimulated by LPS or LPS with αCD40 for 24 hr were sorted using a cell sorter. CD86^high stable mature DCs and CD86^low exhausted DCs were purified. (c) IL-10 and IL-12p40 production by each BM-DC phenotype was determined by ELISA: supernatants of 6-hr cultures of fluorescence-activated cell sorter-purified stable mature DCs (M), exhausted DCs (Ex), immature DCs (Im) and transient mature DCs (tM), respectively, were analysed (n.d., not detected).