Skip to main content
. 2007 Jan;120(1):28–37. doi: 10.1111/j.1365-2567.2006.02474.x

Figure 5.

Figure 5

Efficacy of in vivo cytotoxic T lymphocyte (CTL) induction by several dendritic cell (DC) phenotypes.(a) Protocol for in vivo CTL induction by DC inoculation: 1 week after antigen-pulsed DC immunization, target cells were inoculated intravenously and residual cells in the spleen were analysed 8 hr later. (b) Histograms indicate ovalbumin (OVA)-specific killing in B6 mice immunized with OVA protein-pulsed exhausted DCs (Ex), transient mature DCs (tM) or stable mature DCs (M) (5 × 104/mouse). The bar graph shows the average reduction rate of OVA257-264-target cells (decreased number of OVA257-264-pulsed target cells/decreased number of nucleoprotein (NP)366-374-pulsed target cells) in the spleen from mice immunized with Ex, tM or M. (c) Bone marrow DCs derived from IL-10-deficient (IL-10–/–) or wild type (IL-10+/+) mice were stimulated with lipopolysaccharide in the presence of OVA protein (1 mg/ml) for 3·5 hr. B6 mice were immunized by those DCs, after which in vivo CTL activity was estimated.