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. 2007 Jan;120(1):57–65. doi: 10.1111/j.1365-2567.2006.02477.x

Figure 3.

Figure 3

Proliferation of workshop cluster 1+ (WC1+) γδ T cells in response to cytokine stimulation. Peripheral blood mononuclear cells (PBMCs) were isolated and labelled with carboxy-fluorescein diacetatesuccinimidyl ester (CFDA-SE) according to the kit instructions. The cells were cultured in the same way as for Figure 1 with different combinations of interleukin (IL)-2, IL-15, IL-18 and IL-12 added, and were then fixed in 1% paraformaldehyde, permeabilized and stained for intracellular interferon (IFN)-γ (CC302-PE conjugate) and the WC1 antigen (CC39-Alexa Fluor 647 conjugate). Cytokine combinations used were (a) medium; (b) IL-2, IL-12, IL-15, IL-18; (c) IL-12, IL-15, IL-18, and (d) IL-12, IL-18. The PBMCs were gated on WC1+ cells. The quadrants were set according to the medium control staining. (e) Three populations of CFDA-SE-labelled WC1+ cells were identified by applying markers to a histogram of the WC1+ cells shown in (b).