Figure 4.

Production of interferon (IFN)-γ by purified workshop cluster 1⁺ (WC1⁺) γδ T cells. (a) Intracellular IFN-γ expression was measured using purified WC1⁺ cells. WC1⁺ cells were cultured and stained as in Figure 1. One representative dot plot is shown. (b) WC1⁺γδ T cells were stimulated with interleukin (IL)-12 and IL-18 or medium and cultured for 46 hr. IFN-γ production was measured by enzyme-linked immunosorbent assay. Stimulations were set up as four biological repeats per animal per condition and each was assayed in triplicate. Mean values ± standard error are indicated.