Table 1.
Cytokine requirements for interferon (IFN)-γ production by workshop cluster 1+ (WC1+) γδ T cells. Peripheral blood mononuclear cells (PBMCs) were cultured for 46 hr in the presence of different combinations of cytokines with Brefeldin A added for the final 16 hr. Cells were fixed in paraformaldehyde, permeabilized and stained for intracellular IFN-γ (monoclonal antibody, mAb, 6Η5) and the WC1 antigen (mAb CC39). Results are means of three animals and are expressed as the percentage of the total WC1+ population
| Cytokine combination | Percentage of WC1+ cells that are IFN-γ+ |
|---|---|
| IL-12 + IL-18 + IL-15 + IL-2 | 7·9 ± 3·91 |
| IL-12 + IL-18 + IL-15 | 6·2 ± 2·61 |
| IL-12 + IL-18 | 3·9 ± 2·21 |
| IL-12 + IL-15 | 2·7 ± 1·01 |
| IL-12 + IL-2 | 1·2 ± 0·41 |
| IL-2 + IL-15 | 0·9 ± 0·21 |
| IL-18 + IL-15 | 0·4 ± 0·3 |
| IL-18 + IL-2 | 0·3 ± 0·1 |
| IL-12 | 1·3 ± 0·31 |
| IL-18 | 0·2 ± 0·1 |
| IL-2 | 0·8 ± 0·3 |
| IL-15 | 0·2 ± 0·2 |
| No cytokine added | 0·3 ± 0·3 |
1
Differs significantly (P ≤ 0·05) from medium control.