Table 4.

Interferon (IFN)-γ production by blood and lymph node-derived workshop cluster 1⁺ (WC1⁺) γδ T cells. WC1⁺γδ T cells from peripheral blood mononuclear cells (PBMCs) and prescapular lymph nodes (PSLNs) in 1-month-old animals were cultured for 46 hr in the presence of interleukin (IL)-12 and IL-18 at optimized dilutions, with Brefeldin A added for the final 16 hr. Cells were fixed in paraformaldehyde, permeabilized and stained for intracellular IFN-γ (monoclonal antibody, mAb, 6Η5) and WC1 antigen (mAb CC39)

Animal	% WC1+/IFN-γ+ (PSLN)	% WC1+/IFN-γ+ (PBMC)
476	4·1	0·5
918	1·6	0·4
919	2·5	1·6
921	2·1	0·3
915	13·6	2·2
917	5·7	1·3
Mean/SD	4·9 ± 4·5	1·0 ± 0·7

P = 0·06.