Figure 4.

T-cell effector functions of the BV6⁺ T-cell clone. (a) Cytokines (IFN-γ and IL-4) were measured from supernatants of T cells stimulated with antigen (M. leprae or M. tuberculosis) or media. The data are presented as the mean of triplicate wells and are representative of three experiments. (b) Cytolytic analysis of the BV6⁺ T-cell clone. Leprosy lesion T-cell clones can affect cytolytic activity against M. leprae-pulsed APCs as measured in a ⁵¹Cr-release assay. The data are representative of four separate experiments. (c) T cells were incubated with strontium (18 hr) to degranulate cytolytic mediators or with anti-Fas antibody to prevent Fas–FasL interactions. Monocyte-derived dendritic cells were then incubated with T cells. Cytolytic activity of the T-cell clones measured by a ⁵¹Cr-release assay. The data are representative of three experiments. (d) Two-colour immunofluorescent cell labelling of the BV6⁺ T-cell clone reveals that both granulysin and perforin co-localize in individual cells (white arrows).