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Cytokine secretion induced by DNA/Lipofectin complex from DC2.4 cells. The cells were incubated with EC DNA, pDNA, or CT DNA/Lipofectin complex (5 µl Lipofectin per 1 µg DNA). After a 2 hr incubation, liposomes were removed and fresh growth medium was added to the cells. The supernatants were collected 8 h after the incubation with liposomes. The amount of TNF-α and IL-6 secreted from the cells was determined by ELISA. Each result represents the mean ± SD (n = 3).
