Table 1.

Quantitative measure of the dynamics of CD4⁺ T memory (Tm) cell proliferation

	CD4+ Tm cells maintained with
	Monocytes and IL-7/IL-15 [day 5 (day 7)]		MDCs and IL-7/IL-15 [day 5 (day 7)]
	Proliferative precursors	Mitotic events	Proliferative precursors	Mitotic events
Co-culture	553 ± 187	675 ± 170	7050 ± 282	9672 ± 108
(n = 3)	(2340 ± 786)	(2742 ± 761)	(40491 ± 2925)	(84772 ± 2478)
Transwell	619 ± 137	656 ± 131	665 ± 96*	778 ± 86*
(n = 3)	(1506 ± 279)	(1914 ± 441)	(1582 ± 284*)	(1926 ± 323*)

CD4⁺ Tm cells were maintained with monocytes or myeloid dendritic cells (MDCs) and interleukin (IL)-7/IL-15 in co-culture or transwell assay. The numbers of proliferative precursors and mitotic events⁹ recovered at day 5 or day 7 of culture were obtained by gating on live CD45⁺ CD3⁺ gate and quantified using the TruCOUNT^TM bead assay (see Fig. 1). Data are expressed as the mean value ± standard error of the mean.

^*Values from co-culture and transwell assays differed significantly.