Figure 5.

Simvastatin reduces the cellularity in cervical lymph nodes. Histological analysis of the cervical lymph node cellularity in C57BL/6 wild-type mice, which were daily treated i.p. with simvastatin (b, c black bar) or the ethanol alone (a, c white bar). Cervical lymph nodes were harvested on day 10, stored overnight in 4% formaldehyde and embedded into paraffin. Midline cross-sections of the identical thickness of 4 μm were stained simultaneously with haematoxylin and central high-power fields were recorded on tape, using a defined light source. The relative area of haematoxylin-stained cell nuclei was determined by means of a computer-assisted image analysis program and is given in per cent. Data are mean values ± SEM, n = 6 or n = 7, *P < 0·05 versus ethanol-treated C57BL/6 wild-type mice. Scale bar represents 30 μm (a,b).