Figure 5.

(a) Proliferation of Ab₂ immune splenocytes on in vitro restimulation with purified Ab₁, H protein, H-specific synthetic peptides [H-1, H-2], V_H peptide and V_L peptide (10 μg/ml) derived from the homology regions. The data shown are the mean counts/min of triplicate wells. (b) IL-2 and IFN-γ production by in vitro restimulated Ab₂ immune splenocytes in response to stimulation by full-length antigen and antigen-derived peptides (8 μg/ml). The results shown are representative of three independent experiments.