Table 2.
Generation of memory B-cell response by anti-idiotypic B cells as detected by measuring the amount of secreted antibody1
Antigen used for booster immunization | Antibody titre (× 103)2 |
---|---|
H protein | > 16 |
Anti-idiotypic B cells | > 64 |
Idiotypic B cells | 32 |
Irrelevant B cells | < 2 |
None | < 2 |
Dilutions of sera collected from mice (as mentioned in legend of Fig. 6) were reacted with antigen (200 μ g/well of H protein).
Data shown as titre are from the average optical density of triplicate wells normalized with readings for pre-immune sera.