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. 2007 Apr;120(4):467–485. doi: 10.1111/j.1365-2567.2006.02534.x

Figure 3.

Figure 3

CXCR3–ligand interaction induces rapid protein tyrosine phosphorylation in JE6.1/CXCR3 cells. (a) Jurkat T cells stably expressing CXCR3 (JE6.1/CXCR3) were incubated for 5 min on ice in 0·001% BSA/RPMI alone (lane 1) or in 0·001% BSA/RPMI containing 0. 3 nm CXCL9, CXCL10 or CXCL11 (lanes 2–4). After incubation, the cells were moved to a 37° water bath for 5 min. Proteins in whole cell lysates were separated by SDS–PAGE, transferred to membranes, and immunoblotted with anti-phosphotyrosine mAb. (b) Time–course study of CXCR3-induced protein tyrosine phosphorylation. Cells were stimulated for the indicated time with CXCL10 as described in (a) and protein tyrosine phosphorylation was analysed as in (a).