Table 1.
Oligonucleotide sequence | |
---|---|
Oligonucleotide primers for PCR amplification of CD40L cDNA | |
Sense | 5′-gccagaagataccatttcaa |
Antisense | 5′-cttatgacatgtgccgcaatt |
Sense | 5′-aaggtgatcagaatcctca |
Antisense | 5′-ctgcaaggtgacactgttc |
Oligonucleotide primers for PCR amplification of CD40L promotor | |
Sense | 5′-actggggagagcattcagg |
Antisense | 5′-tctgccaagtaggtggcttc |
Sense | 5′-ccaaagcctctgacttgactg |
Antisense | 5′-tgcacctgtcttgcttatcct |
Sense | 5′-gtgtcaaatttcttccatgcac |
Antisense | 5′-tgactggtgtcccatcaact |
Sense | 5′-gccaggctttcattgagtttag |
Antisense | 5′-gcagaggcagcatgagaag |
Oligonucleotides used as primers for amplification of CD40L 3′ untranslated region and poly A site | |
Sense | 5′-gcaaatacccacagttccgc |
Antisense | 5′-gggacagggtggaaagaagag |
Sense | 5′-acacagagtcaggccgttgc |
Antisense | 5′-tgtcacagacttccagaaagtgtg |
Sense | 5′-aagcagcaaccccactgat |
Antisense | 5′-tctttccccaacctggctg |