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. 2007 May;121(1):105–112. doi: 10.1111/j.1365-2567.2007.02543.x

Figure 2.

Figure 2

Cell surface expressions of SCTs. (a) Flow cytometry analysis of cell surface expression of SCTs. The pcDNA3.0-SCT constructs were transiently transfected to NIH-3T3 cells. Cell surface expression was determined by FITC-labelled anti-HLA-A2 antibody (clone BB7.2). (b) Confocal microscopic detection of the cell surface expression of SCTs on NIH-3T3 cells. After transient transfection, NIH-3T3 cells were stained with FITC-labelled HLA-A2 antibody (clone BB7.2) and analysed using confocal microscopy. NIH-3T3 (negative control), NIH-3T3-pcDNA-SCT-HIVgag (NIH-3T3 cells transfected with pcDNA3.0-SCT-HIVgag).