Skip to main content
. 2007 May;121(1):105–112. doi: 10.1111/j.1365-2567.2007.02543.x

Figure 4.

Figure 4

DNA vaccine with SCT constructs elicits epitope-specific CTL responses in HLA-A2.1/Kb transgenic mice. (a) Four groups of HLA-A2.1/Kb transgenic mice, each contain four to eight mice, were vaccinated with 100 μg pcDNA-3.0-SCTs intramuscularly at 2-week intervals for three injections. Splenocytes were dissected 2 weeks after the last vaccination. Epitope-specific IFN-γ-releasing cells were counted by ELISPOT experiments. pcDNA3.0-SCT vaccines were able to prime CD8+ T-cell responses with statistical significance (P = 0·002, P = 0·009 and P = 0·007 for C18−27, C107−115 and HIV gag, respectively, compared to pcDNA3.0 control group vaccination; SFC, spot-forming cells). (b) Splenocytes were prestimulated with 10 μg/ml epitopes for 2 days and then tested by flow cytometry using FITC-CD8a and APC-IFN-γ antibodies. CD8+ IFN-γ-expressing cells were gated against CD8+ T cells.