Figure 6.

Binding of various CRP forms to human CD binding protein (C4BP). (a) ELISA plates were coated directly with nCRP, Cr-mCRP, urea-mCRP or with nCRP complexed to PC-KLH at 2 μg/ml concentration. PC-KLH-coated wells and uncoated wells were used as controls. Increasing amounts of purified human C4BP were added and detected by anti-C4BP antibodies. (b) ELISA plates were coated with human C4BP (2 μg/ml) and incubated with either nCRP or Cr-mCRP (10 μg/ml). Monoclonal antibodies specific of the native (1D6) or modified (9C9 and CRP-8) CRP conformation were used to determine the amounts of CRP bound to the plate. The data shown represent the means ± SD of two experiments.