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Analysis by surface plasmon resonance spectroscopy of the interaction between native CRP or modified CRP and immobilized C4BP. Sensor chips were coated with C4BP (5230 RU) as described in the Materials and methods. Sixty microlitres of nCRP (100 nm), or Cr-mCRP (25–200 nm) were injected in the running buffer (0·01 m HEPES, 0·15 m NaCl, 0·67 mm CaCl2, 0·005% v/v surfactant P20, pH 7·4) at a flow rate of 20 μl/min. Resonance units are indicated as a function of time.
