Table 2.
Kinetic and dissociation constants for the interaction of C1q and C1q-GR with immobilized native CRP or modified CRP as determined by surface plasmon resonance
| kon(/m second) | koff(/second) | KD(nm) | Rmax(RU) | |
|---|---|---|---|---|
| Immobilized nCRP (4300 RU) | ||||
| C1q | 7·4 × 105 | 1·1 × 10−3 | 1·45 | 119 |
| C1q-GR | 3·5 × 103 | 2·4 × 10−3 | 680 | 103 |
| Immobilized Cr-mCRP (5500 RU) | ||||
| C1q | 1·5 × 106 | 2·9 × 10−3 | 1·95 | 291 |
| C1q-GR | 5·1 × 104 | 1·6 × 10−3 | 31·6 | 310 |
Binding of C1q and of C1q GR was measured over 4300 and 5500 RU of immobilized nCRP and Cr-mCRP, at a flow rate of 20 μl/min, in 145 mm NaCl, 2 mm CaCl2, 50 mm triethanolamine–HCl containing 0·005% surfactant P20.