Figure 2.

PPAR-γ agonists inhibit ICAM-1 mRNA expression in RSV-infected A549 cells. The cellular amount of mRNA encoding for ICAM-1 was quantitatively determined by real-time RT–PCR 24 hr postinfection. (a) Cells were pretreated with ciglitazone (20–40 µm), troglitazone (20–40 µm), 15d-PGJ₂ (5–20 µm) or medium (control) for 30 min. (b) Cells were pretreated with Fmoc-Leu (200–400 µm) or vehicle (DMSO, 0·1–0·3% (v/v)) for 30 min. Then, cells were infected with RSV (m.o.i. = 3) and cultured in the presence of the agonists for 24 hr. Results are means ± SEM (n = 6); *P < 0·05 versus non-treated RSV-infected cells; **P < 0·01 versus non-treated RSV-infected cells.