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. 2007 May;121(1):71–81. doi: 10.1111/j.1365-2567.2006.02539.x

Figure 6.

Figure 6

PPAR-γ agonists inhibit the adhesion of monocytic cells (U937) to RSV-infected epithelial cell monolayers (A549). (a) Following RSV infection (m.o.i. = 1) the monolayers were incubated with the following PPAR-γ agonists: ciglitazone (20 µm), troglitazone (20 µm), Fmoc-Leu (200 µm),15d-PGJ2 (10 µm), DMSO (0·1% v/v)), or medium for 36 hr. (b) Role of ICAM-1 and β2 integrins in the adhesion process of U937 cells to A549 cell monolayers. Cells were preincubated with blocking antibodies specific for ICAM-1 (20 µg/ml), CD11a (20 µg/ml), CD11b (20 µg/ml), CD18 (20 µg/ml) or IgG isotype controls (20 µg/ml IgG1 + 20 µg/ml IgG2a). The adhesion of calcein-AM-labelled U937 cells to A549 monolayers was determined by measuring cell-bound fluorescence. The fluorescence signal of U937 cells adherent to RSV-infected monolayer was adjusted to 100% and data are expressed as percent of this value. Results are means ± SEM (n = 3, performed in quadruplicate); *P < 0·01 versus non-treated, RSV-infected cells.