Figure 3.

Liposome–mediated association of OVA to BM-DCs. (a) OVA acquisition is concentration-dependent: BM-DCs were cultured for 1 hr with different concentrations of OVA-fluorescein with (+DDA) or without (–DDA) DDA liposomes. (b) DDA liposomes enhance the rate of OVA acquisition: varying incubation time, BM-DCs were pulsed with 5 μg/ml OVA with (black columns) or without (grey columns) liposomes. The amount of OVA associated with live CD11c⁺ cells was determined by flow cytometry. Data are representative of two experiments each.