Figure 4.

Liposome-mediated uptake of OVA by BM-DCs. BM-DCs were cultured for 30 min at 37° or 4° with 5 μg/ml OVA-AlexaFluor488 alone (middle panel) or adsorbed to DDA liposomes (lower panel). Cells were washed in either PBS or ethanoic acid buffer to remove surface-associated OVA. The amount of OVA associated with CD11c⁺ cells was determined by flow cytometry. The corresponding geometric MFI values are given in the top right-hand corner of each histogram. The top panel shows the unstimulated control. Data are representative of three experiments.