Figure 6.

Liposome-mediated uptake of OVA by peritoneal exudate cells. (a) Mice were injected with the indicated dose of OVA-AlexaFluor488 alone (–DDA) or adsorbed to 250 μg DDA liposomes (+DDA) and peritoneal exudate cells were harvested 2 hr later. Cells were washed in ethanoic acid buffer to remove surface-associated OVA. The percentage of cells with internalized OVA was determined by flow cytometry. Bars show the means of three individual mice per group and the error bars represent the standard error of the means (SEM). (b) The distribution of OVA uptake by the cells from one representative mouse from each group. The top panel is OVA injected alone, the lower panel is OVA adsorbed to liposomes. Where adsorption onto the liposomes statistically significantly enhanced the uptake of OVA, this has been indicated: *P < 0·05 and **P < 0·01. Data are representative of two experiments.