Figure 8.

DDA-mediated targeting of OVA to BM-DCs is inhibited by heparin. BM-DCs were pretreated for 45 min at 37° with or without 250 IU/ml heparin before stimulation for 30 min with OVA-AlexaFluor488 alone or adsorbed to DDA liposomes. Unstimulated cells are shown as control. The amount of OVA associated with live CD11c⁺ cells was determined by flow cytometry. Bars show the mean and error bars represent the standard deviation of triplicate determinations. The triplicate determinations of the heparin-treated groups were so similar that the error bars fall within the edge of the bars.