Table 3.
The reactivity of B cells, monocytes and polymorphonuclear (PMN) cells with KB61 or 2B6 is independent of the alloform of CD32A1
| B cells | Monocytes | PMN cells | |
|---|---|---|---|
| CD32A-R/R131 (n = 18) | |||
| KB61 | 99 ± 1 | 98 ± 2 | 99 ± 1 |
| 2B6 | 95 ± 4 | 21 ± 18 | 4 ± 4 |
| CD32A-H/H131 (n = 8) | |||
| KB61 | 97 ± 3 | 97 ± 1 | 99 ± 1 |
| 2B6 | 94 ± 4 | 26 ± 14 | 4 ± 4 |
| CD32A-H/R131 (n = 20) | |||
| KB61 | 99 ± 1 | 97 ± 2 | 99 ± 1 |
| 2B6 | 95 ± 3 | 21 ± 15 | 4 ± 5 |
1
Forty-six of the 48 healthy donors shown in Fig. 4(c) were genotyped for 131 polymorphism of CD32A as described in the Materials and Methods. B lymphocytes were defined by costaining with anti-CD20-PE while monocytes and PMN cells were identified by using FSC versus SSC plots. Cells were stained with either KB61-FITC or 2B6-FITC and threshold levels were determined by staining cells with an isotype-matched, FITC-labelled control mAb (mIgG1-FITC). The percentage of CD32-positive cells (± SD) in each gate is indicated.