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Immunofluorescence studies and Western blotting (WB) showed that PBMC lack Dsg1 and Dsg3. Anti-Dsg1 and anti-Dsg3 were first probed separately, then the results were summarized in the same blot by incubating PVDF filters with both antibodies. Keratinocyte lysates (ker) served as a positive control. Con, negative control PBMC obtained by using only FITC-conjugated anti-human antibodies. The figures are representative of three independent experiments.
