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. 2007 Jul;121(3):377–382. doi: 10.1111/j.1365-2567.2007.02585.x

Figure 3.

Figure 3

(a) Ponceau staining of PVDF membranes blotted with immunocomplexes shows a slight 130 000 band immunoprecipitated by PV (n = 4) but not control sera. (b) By increasing the contrast of the surface of interest (adobe Photoshop), the 130 000 band (arrow) clearly appeared in lanes 1–4 (PV sera, asterisks) but not in lane 5 (control). (c) Combined immunoprecipitation (IP)-Western blotting (WB) with PV IgG demonstrated that IgG from PV sera recognized a 130 000 MW protein. (d) Pre-incubation of PV IgG (primary antibody) with gel-pure 130 000 MW protein(s) from keratinocyte extracts strongly reduced the ability of PV IgG to bind the 130 000 PBMC antigen. (e) Immunoprecipitation of keratinocyte extracts with H-145 anti-Dsg3 (positive control), Nh or PV IgG followed by Western blotting against Dsg3 revealed that immunocomplexes other than positive controls did not contain Dsg3. Results were confirmed in at least two experiments carried out independently.